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Journal: The Journal of Reproduction and Development
Article Title: Preclinical evaluation of a new cryopreservation container for a limited number of human spermatozoa
doi: 10.1262/jrd.2017-086
Figure Lengend Snippet: CMA3 staining of fresh or freeze-thawed murine spermatozoa. CMA3-positive murine spermatozoa appear bright green, while CMA3-negative spermatozoa appear bright yellow. Bar: 20 µm. Picture of fresh spermatozoa (A) and spermatozoa in the straw (B) and the new container (C). Staining was repeated three times in the three groups (n = 3). The yellow color indicates non-damaged sperm and the green color indicates sperm with damaged nuclei. Spermatozoa with green heads were defined as CMA3-positive (∆) and those with yellow heads were defined as CMA3-negative (▲).
Article Snippet: Thereafter, the suspension was smeared on glass slides (S9441, Matsunami Glass) and smears were treated for 20 min with 200 µl of
Techniques: Staining